Chippenham Town striker David Pratt is thought to have set a new world record after being sent off only three seconds into his side's loss at Bashley.
Pratt, 21, lunged at Bashley midfielder Chris Knowles straight from the kick-off and received his marching orders from referee Justin Amey.
Bashley went on to win the Southern Premier Division match 2-1.
Knowles was able to continue playing after the tackle.
Bologna's Giuseppe Lorenzo held the previous record for the quickest red card, at 10 seconds.